| The Effect of Anti-oxidants on Apoptosis Relating Protein Expressions in Mutant Cu/Zn-superoxide Dismutase Motor Neuron Cells |
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Dong Hoon Shin, Manho Kim, Chang Seok Oh, Jaehyup Kim, Kwang Woo Lee, Choong Ik Cha, Wang Jae Lee, Young Soo Lee |
1Department of Anatomy, Seoul National University College of Medicine, Seoul, Korea.
2Department of Neurology, Seoul National University College of Medicine, Seoul, Korea.
3Department of Internal Medicine, Asan Medical Center, University of Ulsan, Seoul, Korea. |
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| Abstract |
BACKGROUND
S: Free radical theory showed that aging might be correlating with the accumulation of oxidative damage into biomolecules of animals. Since the process was also observed in some neurodegenerative disease, the study on the subject could improve our understanding of the aging process. In this regard, our study laid focus on the effect of various anti-oxidants on the cells including mutant Cu/Zn-superoxide dismutase, which was reported to be deficient in various diseases or aging processes.
METHODS
We tested retinol, Vitamin C, E and coenzyme Q10 on mutant Cu/Zn-superoxide dismutase(A4V) motor neuron cells using immunocytochemical study and accompanying statistical analysis.
RESULTS
During our trial on the hydrogen peroxide treatment on A4V mutant cells, Vitamin A and C did not show any defensive effect on oxidative stress induced A4V cells while Vitamin E and coenzyme Q10 exhibited meaningful inhibitory effect against apoptosis relating protein expression.
CONCLUSION
In case of oxidative damages induced by Cu/Zn-superoxide dismutase deficiencies, the protective role of some anti-oxidants like Vitamin E or coenzyme Q10 should be positively considered in forthcoming studies. |
| Key Words:
Aging, Amyotrophic lateral sclerosis, Hydrogen peroxide, Apoptosis, Immunocytochemistry |
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