| Role of Cytosolic Calcium Ion and Expression of Proliferation Responsive Gene of Cultured Medial Smooth Muscle Cells Obtained from rat Aortas with Atherosclerotic Changes Induced by High Cholesterol Feeding |
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Hyung Joon Yoo |
| 노고콜레스테롤식이로 죽상경화를 유발한 당뇨병 유도백서의 대동맥에서 채취 배양한 혈관평활근 세포의 증식에서 페포질 칼슘 이온의 역할과 증식반응 유전자 발현 |
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| Abstract |
BACKGROUND
Vascular smooth muscle cells (VSMCs) proliferation is an important process in the pathogenesis of atherosclerosis. VSMCs proliferation is closedly related to the cytosolic calcium flux via L-type voltage dependent calcium channel.
OBJECTIVES: To investigate the role of cytosolic Ca(2+) in the proliferation of VSMCs.
METHODS
The proliferation of aortic vascular smooth muscle cells of rats(1% cholesterol diet fed rats and general diet fed ones), acquired by enzymatic method. Cell counting, and calcium agonists(Bay K 8644 10(-6)M) or calcium antagonists(verapamil 10(-6)M). Cytosolic calcium ion was measured with Fura 2 method. Calcium channel gene expresssion was detected with RT-PCR method.
RESULTS
Masson Trichrome staining shows more disturbed cell lining, more VSMCs, more degenerated media, more collagen laydown, and more adventitial fat in the aorta of cholesterol-fed rats than in that of general diet-fed ones. VSMCs of cholesterol fed-rats were moreproliferated than those of general diet fed-ones. Bay K 8644 enhanced VSMCs proliferation of both groups. Verapamil blocked the incremental effects induced by Bay K 8644. Expression of calcium channel gene was enhanced by Bay K 8644 and was reversed by verapamil.
CONCLUSION
The enhanced proliferation of atherosclerotic VSMCs is associated with the increment in cytosolic calcium, which is accomplshed through the expression of L-type voltage dependent calcium channel. |
| Key Words:
Diabetes, Atherosclerosis, Cytosolic calcium, Calcium channel gene |
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