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Journal of the Korean Geriatrics Society 2008;12(3):146-152.
Published online September 30, 2008.
Alteration of Antioxidant Enzyme Gene Expression with Aging and Exercise in Fisher Rats
Bong Jun Cho, Jun Tae Kim, You Jin Lee, Sung Hoon Yu, Sung Hee Choi, Soo Lim, Jae Young Lim, Hyuk Jae Chang, Young Joo Park, Hak Chul Jang
노화와 운동이 백서의 항산화 유전자 발현에 미치는 영향
It has been reported that radical oxygen species(ROS) production increased drastically during ageing. This increase in ROS had negative effects on many age-related diseases. In this study, we investigated the effects of ageing and exercise on antioxidant gene expression in Fisher rats and the mechanism of chronic exercise on ROS generation related to ageing.
After a standard diet, young and old male Fischer rats were assigned to sedentary control groups(young control group: YC, old control group: OC) and exercise training groups(young exercised group: YE, old exercised group: OE). After a 12-week treadmill exercise training in the exercise training groups, antioxidant gene expre- ssion(catalase, glutathione peroxidase) in the liver and muscle was measured by RT-PCR(reverse transcription- polymerase chain reaction).
Liver catalase mRNA expression was lower in the old group compared with that in the young group. However, this remained unchanged post-exercise. Liver glutathione peroxidase mRNA expression was not different between the young and old groups and remained unchanged in both these groups. The expression of catalase mRNA in the soleus muscle was significantly lower in the old control rats compared with that in the young rats. Exercise sig- nificantly increased catalase mRNA expression in the soleus in both the young and old rats. The expression of gluta- thione peroxidase mRNA in the soleus was lower in the old control rats than in the young. Exercise significantly increased glutathione peroxidase mRNA expression in both young and old rats.
This study showed that chronic exercise could be an important contributor to the recovery in the decline of certain antioxidant gene expressions in both young and aged rats. Long-term exercise may posi- tively affect metabolic diseases by modulating antioxidant gene expression.
Key Words: Aging, Exercise, Reactive Oxygen Species(ROS), Catalase, Glutathione peroxidase
초 록

연구배경: 활성산소종(reactive oxygen species, ROS)의 증가는 체내 여러 가지 대사조절 과정에 좋지 않은 영향을 미치는데, 최근 노화가 진행함에 따라 이러한 활성산소종 경로가 증가됨이 보고되고 있다. 본 연구에서는 젊은 백서와 고령의 백서에서 활성산소종을 조절하는 항산화 유전자의 발현을 살펴보고, 이에 미치는 만성 운동의 효과를 살펴봄으로써, 노화와 연관된 활성산소종 증가 기전의 일단을 살펴보고자 하였다.

방법: 백서를 표준 식이를 이용하여 기른 후 젊은군(12주령)과 노령군(88주령)으로 나누고, 각 군을 대조군(젊은 대조군; YC, 노령 대조군; OC)과 운동군(젊은 운동군; YE, 노령 운동군; OE)으로 분류하여 운동군은 12주간의 답차 운동(YE: 하루 45-60분, 15m/min; OE: 하루 20~30분, 4m/min)을 실시 하였다. 항산화 유전자 발현을 알아 보기 위하여, 간, 근육 조직의 catalase와 glutathione peroxidase의 mRNA 발현 정도를 RT-PCR(Reverse transcription-polymerase chain reaction) 방법을 이용하여 알아 보았다.

결과: 젊은군(OC)의 경우 노령군(YC)에 비하여 간 조직에서 catalase mRNA 발현이 감소 되어 있었으며, gluta- thione peroxidase의 mRNA발현에는 별다른 변화가 없었다. 가자미근에서의 catalase와glutathione peroxidase mRNA의 발현은 노령군(OC)의 경우 젊은군(YC)에 비하여 발현이 유의하게 감소되었으며, 운동을 통해 노령군(OE)과 젊은군(YE) 모두 catalase와 glutathione peroxidase의 mRNA 발현이 유의하게 증가하였다.

결론: 장기적인 운동이 노화에서 감소된 항산화 유전자 발현을 회복시키며, 이는 장기적인 운동이 노화에 따른 대사 이상을 일부 회복시킬 가능성을 시사한다.

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