| Distribution Change of WD40 Repeat Protein 1 in Artificially Induced Senescent PC12 Cells |
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Dong Hoon Shin, Chang Seok Oh, Eunju Lee, Seung Ha Oh, Young Soo Lee |
1Department of Internal Medicine, Asan Medical Center University of Ulsan College of Medicine, Seoul, Korea. yslgerit@amc.seoul.kr
2Department of Anatomy, and 3Otorhinolaryngology, Seoul National University College of Medicine, Seoul, Korea. |
| WD40 repeat protein 1의 PC12 갈색세포종 노화유발 세포에서의 분포 변화 |
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| Abstract |
Background
WDR1 is thought to be correlating with polymerization and depolymerization of actin protein. Though WDR1 was found to be within nucleus, in which actin could not be present by previous studies, the exact distribution pattern of WDR1 protein under various circumstances was not elucidated up to the present time. In this regard, we tried to see a change in the distribution of WDR1 protein within artificially induced senescent PC 12 pheochromocytoma cells for the first time.
Methods
PC12 pheochromocytoma cells (ATCC CRL-1721) were grown in the culture media including 1 micrometer 3'-Azido-3'-deoxythymidine (AZT, Sigma-Aldrich, USA). The senescence of the cells was confirmed by senescence detection kit (Calbiochem, San Diego, CA). Immunocytochemical study by using WDR1 antibody was also performed in the cells treated with AZT during 0, 75 and 153 days.
Results
WDR1 protein was mainly observed within the cytoplasm of the cells not treated with AZT. However, the distribution of the same protein was changed into the nucleus after 153 day-AZT treatment.
Conclusion
The distribution of WDR1 protein was changed into nucleus in the artificially senescent PC12 cells. |
| Key Words:
WD40 repeat protein 1, PC12, Senescence, 3'-Azido-3'-deoxythymidine |
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