| Effects of Ca2+ Channel Blockers on Expression of L-type Voltage Dependent Ca2+ Channels in Vascular Smooth Muscle Cells Obtained from the Aorta of Diabetic Rats |
|
Hyung Joon Yoo |
| 당뇨병 백서 대동맥 혈관평활근세포 L-type Voltage Dependent Ca2+ Channels 유전자 발현에 대한 Ca2+ Channel Blockers의 작용 |
|
|
|
|
|
|
| Abstract |
BACKGROUND
The proliferation of vascular smooth muscle cells(VSMCs) is an important process in the pathogenesis of atherosclerosis. VSMCs proliferation is closedly related to the cytosolic calcium flux via L-type voltage dependent calcium channel.
OBJECTIVES: To investigate the role of cytosolic Ca2+ in the proliferation of VSMCs.
METHODS
The proliferation of aortic vascular smooth muscle cells of streptozotocin induced diabetic rats, acquired by enzymatic method. Cell counting, and calcium agonists(Bay K 8644 10(-6)M) or calcium antagonists(verapamil 10(-6)M). Cytosolic calcium ion was measured with Fura 2 method. Calcium channel gene expression was detected with RT-PCR method.
RESULTS
VSMCs of diabetic rats were more proliferated than those of nondiabetic ones. Bay K 8644 enhanced VSMCs proliferation of both groups. Verapamil blocked the incremental effects induced by Bay K 8644. Expression of calcium channel gene was enhanced by Bay K 8644 and was reversed by vera- pamil.
CONCLUSION
The enhanced proliferation of VSMCs is associated with the increment in cytosolic calcium, which is accompli shed through the expression of L-type voltage dependent calcium channel. |
| Key Words:
Diabetes, Vascular smooth muscle cell, Calcium channel |
|
PDF Links
Full text via DOI
Download Citation
