Proliferation of Cultured Vascular Smooth Muscle Cells(VSMCs) Obtained from Aortas of Insulin Dependent Diabetic Rats

Yoo, Hyung Joon

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Original Article

Journal of the Korean Geriatrics Society 1997;1(1):48-54.
Published online October 30, 1997.

Proliferation of Cultured Vascular Smooth Muscle Cells(VSMCs) Obtained from Aortas of Insulin Dependent Diabetic Rats

Hyung Joon Yoo

Department of Internal Medicine, Hallym University College of Medicine, Seoul, Korea

인슐린 의존성 당뇨병 백서 대동맥으로부터 배양한 중막평활근세포의 증식능

Abstract

BACKGROUND
Vascular smooth muscle cells(VS-MCs) from diabetic animals proliferate more than that from non-diabetic ones. The proliferative ability of cultured VSMCs is changed by the kind of experimental animals, experimental technique and so on. In this study, I used VSMCs from streptozotocin-induced insulin dependent diabetic Sprague Dawley rats and examined the characteristics of aortic VSMCs proliferation from these rats.
METHODS
Using enzyme method I cultured aortic VSMCs. I compared the proliferation between normal rat VSMCS and diabetic ones. I studied the cell growth of VSMCs from diabetic rats as a function of period after streptozotocin injection. I also examined the effects of hyperglycemia and insulin treatment on the proliferation of cultured VSMCs.
RESULTS
Diabetic rats VSMCs proliferated more than non-diabetic ones in streptozotocin-induced insulin dependent diabetic rats. The proliferation of VSMCs was enhanced from 2 weeks after streptozotocin injection. Hyperglycemia enhanced the proliferation and insulin treatment lowered that.
CONCLUSIONS
The proliferation of VSMCs was increased in streptozotocin-induced diabetic rats.

Key Words: Diabetic rat, Streptozotocin, Vascular smooth muscle cell, Proliferati

초 록

배경 : 당뇨병에서의 죽상경화증 발생에 혈관 평활근세포의 증식은 중요한 병태기전의 하나이다. 그러나 그 증식은 여러 조건들에 의해 다양하게 나타난다. 흔히 사용하는 스트렙토조토신 유도 당뇨병 백서의 혈관평활근 세포 증식능의 특성을 파악하고자 본 연구를 실시한다.

방법 : 스트렙토조토신 유도 당뇨병 백서의 대동맥으로부터 효소법을 이용하여 중막 평활근세포를 채취 배양하여 정상 백서 세포의 증식능과 비교하고, 스트렙토조토신 주입 후의 증식능의 변화를 계측하고, 고혈당과 인슐린 치료에 의한 증식능의 변화를 연구하였다.

결과: 당뇨병 백서의 평활근세포는 정상군에 비해 증식능이 증가하였고 고혈당은 증식능을 증가시켰다. 인슐린 치료에 의해 혈당이 정상화되면 증식능이 감소하였다. 스트렙토조토신 주입 후 2주가 지나면서 평활근세포 증식능이 증가하였고 제 4주 후부터는 플라토를 이루었다.

결론 : 스트렙토조토신으로 유도된 당뇨병 백서의 혈관평활근세포의 증식능은 증가하였다.

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